EBOO Therapy and the Transformative Power of the Filtrate: A Game-Changer in Integrative Medicine

By Dr. Mitra Basu Chhillar, M.D.,

Medical Director,

SOMA Wellness Clinic, Mumbai

In the rapidly evolving field of regenerative and integrative medicine, hemofiltration continues to hold its ground as one of the most effective therapeutic tools for reducing systemic inflammation, removing metabolic waste, and eliminating toxic compounds. Among the various extracorporeal techniques available, EBOO therapy (Extracorporeal Blood Oxygenation and Ozonation) stands apart due to its dual-action mechanism—controlled ozone administration combined with real-time filtration of the bloodstream.

While much attention has been given to the benefits of ozone in modulating immunity, improving oxygen delivery, and enhancing antioxidant systems, an underappreciated yet equally crucial component of EBOO therapy is filtration. Different types of high-flux hemofilters have long been validated for their ability to remove inflammatory cytokines, lipid peroxidation products, bacterial endotoxins (LPS), and other immune-disrupting elements.

This filtration becomes particularly powerful when used in conjunction with ozone-resistant dialyzers and ozone-compatible medical-grade tubing, allowing the simultaneous purification and modulation of blood. This makes EBOO not only a highly effective and safe method of ozone delivery via semi-permeable diffusion—thus avoiding direct gas injection—but also a therapeutic filtration procedure that holds both diagnostic and prognostic value.

The Visual Power of the Filtrate: Quasi-Diagnostic and Prognostic

One of the most fascinating and clinically relevant aspects of EBOO therapy is the filtrate that is collected during the session. In a single session, anywhere from 200 ml to over 1 liter of filtrate may be discarded, depending on the flow rate, filter type, and duration. What is observed in this fluid is often nothing short of eye-opening for both clinicians and patients.

Visual inspection reveals a spectrum of filtrate characteristics:

• Clear yellow filtrate: Often seen in healthy or mildly toxic patients, indicating low inflammatory load.
• Cloudy, turbid, or foamy filtrate: Suggestive of lipid peroxides, oxidized LDL, or high cytokine concentrations.
• Brownish or dark filtrate: Reflects hemolysis, oxidative stress, or severe toxin burden.
• Foul-smelling filtrate: Often correlates with VOCs (volatile organic compounds), endotoxemia, or liver dysfunction.

In fact, the appearance, odor, and consistency of the filtrate can offer quasi-diagnostic insights. For example, in a patient with autoimmune disease, the filtrate may be filled with oxidative byproducts, inflammatory markers, and necrotic debris. In cases of chronic fatigue or fibromyalgia, the filtrate often shows turbidity and foamy layers, suggesting underlying mitochondrial and toxic stress.

The filtrate also has prognostic value—with serial EBOO sessions, a progressive lightening and clearing of the filtrate is often observed in recovering patients. Thus, not only is EBOO therapeutic, but the filtrate itself becomes a biological indicator of response to treatment.

Constituents of the EBOO Filtrate: What Are We Removing?

The filtrate removed during EBOO therapy is a potent reflection of the body’s biochemical distress and immune burden. Using high-flux filters, the following categories of substances are typically found in the filtrate:

1. Inflammatory Mediators
   • Cytokines such as TNF-α, IL-1β, IL-6, IL-8
   • Prostaglandins, leukotrienes, and chemokines
2. Oxidized Lipids and Lipoproteins
   • Malondialdehyde (MDA), 4-HNE (4-hydroxynonenal)
   • Oxidized LDL (oxLDL)
   • Advanced lipid oxidation end products (ALEs)
3. Toxic Metabolic Waste
   • Urea, creatinine, uric acid
   • Lactate (especially in mitochondrial dysfunction)
   • Ammonia
4. Bacterial and Pathogen Debris
   • Lipopolysaccharides (LPS)
   • Viral proteins and glycoproteins
   • Fungal wall components (e.g., β-glucans)
5. Exosomes and Senescent Cell Products
   • Apoptotic blebs, cell-free DNA
   • Senescence-associated secretory phenotype (SASP) components
   • Matrix metalloproteinases (MMPs)
6. Environmental Toxins
   • Heavy metals (lead, cadmium, mercury)
   • Pesticide metabolites and VOCs

Each of these compounds has deleterious effects on mitochondrial function, cellular metabolism, immune surveillance, and systemic homeostasis. Removing them, even in small but repeated quantities, produces measurable clinical improvement.

Clinical Significance: Beyond Just Ozonation

While the therapeutic benefits of ozone are well-documented, it’s crucial to understand that EBOO is far more than just ozone delivery. If the goal is merely to increase the NAD+/NADH ratio or modulate Nrf2 pathways, intravenous ozone via major autohemotherapy may suffice.

However, when the clinical objective is to simultaneously detoxify the bloodstream, remove inflammatory drivers, and modulate the immune system at a foundational level, EBOO becomes unparalleled. Its impact is multidimensional:

• Systemic inflammation is reduced through cytokine clearance
• Oxygen delivery is optimized by improving red cell deformability
• Toxin burden is lowered, reducing hepatic and renal load
• Immunity is re-trained by removing LPS and oxidative debris
• Microcirculation is enhanced by eliminating viscosity-altering waste

Furthermore, the biological waste discarded via the filtrate often correlates with symptomatic relief reported by patients: better sleep, reduced brain fog, decreased joint pain, improved mood, and more stable energy levels.

Importantly, without the filtration component, dissolving ozone into the blood alone will not achieve these effects. That is why true EBOO therapy cannot be equated to high-dose ozone alone.

Technical Requirements for Safe and Effective EBOO

To deliver EBOO safely and effectively, the following technical standards must be observed:

1. Ozone Generator
   • Must be CE-certified or FDA-approved
   • Should offer precise concentration control (05–70 μg/mL)
2. Oxygen Source
   • Must be 100% medical-grade oxygen (not concentrators)
3. Ozone-Compatible Tubing and Filter
   • Use of Teflon, silicone, or specific ozone-resistant polymers
   • Filters must be high-flux, biocompatible, and ozone-resistant
4. Trained Personnel
   • Only clinicians trained in ozone medicine and hemofiltration should administer EBOO

Improper equipment or untrained execution can negate the benefits and potentially cause harm. Hence, EBOO should only be offered in qualified clinical settings.

Why Doctors Should Learn EBOO and Patients Should Seek It

If you are a physician working in chronic disease, integrative oncology, autoimmune care, or neurodegenerative medicine, EBOO offers a tool to directly modify the terrain of your patient. It allows you to target inflammation, mitochondrial dysfunction, and toxicity—not just symptoms.

As a patient, especially if you suffer from:

• Chronic fatigue
• Post-COVID syndrome
• Fibromyalgia
• Lyme disease
• Mold exposure
• Autoimmune flares
• Early cognitive decline

…EBOO therapy may help reset your internal biochemistry at the root level.

The transformative power of the filtrate is not just symbolic. It is visible. It is measurable. And it is repeatable. Each session tells a story—of your toxins leaving the body, of your immune system recalibrating, and of your tissues breathing freely again.

References

S. No.	Study / Article	Link
1	Bocci V. Ozone: A New Medical Drug. Springer, 2011.	Springer
2	Elvis AM, Ekta JS. “Ozone therapy: A clinical review.” J Nat Sci Biol Med. 2011;2(1):66–70.	PMC
3	Tylicki L et al. “Cytokine removal in hemofiltration.” Blood Purif. 2001;19(3):215–22.	PubMed
4	Smith RA et al. “Removal of oxidized LDL during extracorporeal treatments.” Clin Nephrol. 2008;70(3):216–21.	PubMed
5	Kucukardali Y et al. “LPS detoxification by extracorporeal purification.” J Int Med Res. 2007;35(5):646–54.	PubMed
6	Ricci Z, Ronco C. “Pathophysiology of sepsis and extracorporeal therapies.” Crit Care Clin. 2020;36(1):55–70.	PubMed
7	Rota C et al. “Lipid peroxidation products in EBOO-treated patients.” Free Radic Res. 2018;52(4):420–430.	PubMed

To learn more about EBOO therapy or to schedule a treatment or training, contact us at:

SOMA Wellness Clinic
A-507, Kohinoor Square, Dadar, Mumbai
www.somawellnessclinic.com

Disclaimer: This article is intended for informational and educational purposes only. It is not a substitute for professional medical advice, diagnosis, or treatment. Do not attempt any medical treatment described herein without direct supervision by a qualified healthcare provider. The author and SOMA Wellness Clinic disclaim all liability for any outcomes resulting from the application of the information provided. Always consult a licensed physician before beginning any new therapy.